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Literature summary for 2.1.1.148 extracted from

  • Graziani, S.; Xia, Y.; Gurnon, J.R.; Van Etten, J.L.; Leduc, D.; Skouloubris, S.; Myllykallio, H.; Liebl, U.
    Functional analysis of FAD-dependent thymidylate synthase ThyX from Paramecium bursaria Chlorella virus-1 (2004), J. Biol. Chem., 279, 54340-54347.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paramecium bursaria Chlorella virus-1

Protein Variants

Protein Variants Comment Organism
E190G no detectable activity Paramecium bursaria Chlorella virus-1

Inhibitors

Inhibitors Comment Organism Structure
10-methyl-5,8-dideazafolate 0.2 mM, 90% inhibition Paramecium bursaria Chlorella virus-1
10-propargylfolate 0.2 mM, 85% inhibition Paramecium bursaria Chlorella virus-1
5-fluorodeoxyuridine 0.05 mM, 95% inhibition Paramecium bursaria Chlorella virus-1
8-aminopurinone deoxyribonucleoside 5'-phosphate 0.05 mM, 50% inhibition Paramecium bursaria Chlorella virus-1
additional information no inhibition by 10-propargyl-5,6-dideazafolate, 10-methylfolate, 8-azapurinone deoxyribonucleoside 5-phosphate Paramecium bursaria Chlorella virus-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
dUMP
-
Paramecium bursaria Chlorella virus-1
0.02
-
5,10-methylenetetrahydrofolate
-
Paramecium bursaria Chlorella virus-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Paramecium bursaria Chlorella virus-1

Organism

Organism UniProt Comment Textmining
Paramecium bursaria Chlorella virus-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paramecium bursaria Chlorella virus-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
Paramecium bursaria Chlorella virus-1 dTMP + tetrahydrofolate + FAD
-
?
additional information oxidation of NAD(P)H is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Paramecium bursaria Chlorella virus-1

Synonyms

Synonyms Comment Organism
thymidylate synthase ThyX
-
Paramecium bursaria Chlorella virus-1
ThyX
-
Paramecium bursaria Chlorella virus-1

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Paramecium bursaria Chlorella virus-1
NADH oxidation of NADH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1
NADPH oxidation of NADPH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1