Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain DAY4.1 | Saccharomyces cerevisiae |
expressed in Saccharomyces cerevisiae strain DAY4.1 | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-homocysteine | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.014 | - |
L-homocysteine | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.084 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.094 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.109 | - |
5-methyltetrahydropteroyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.113 | - |
5-methyltetrahydropteroyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.12 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.129 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography | Candida albicans |
HiTrap heparin column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
29.3 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 5.6fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae |
additional information | - |
42.11 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 11.7fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Saccharomyces cerevisiae | tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Saccharomyces cerevisiae | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Saccharomyces cerevisiae | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
r | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
r | |
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine | - |
Saccharomyces cerevisiae | tetrahydropteroyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine | - |
Saccharomyces cerevisiae | tetrahydropteroyltri-L-glutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyltri-L-glutamate + L-methionine | - |
? | |
additional information | does not use 5-methyltetrahydropteroylglutamate as a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | does not use 5-methyltetrahydropteroylglutamate as a substrate | Candida albicans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cobalamin-independent methionine synthase | - |
Saccharomyces cerevisiae |
cobalamin-independent methionine synthase | - |
Candida albicans |
Met6p | - |
Saccharomyces cerevisiae |
Met6p | - |
Candida albicans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
5-methyltetrahydropteroyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.33 | - |
L-homocysteine | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.35 | - |
5-methyltetrahydropteroyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.4 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.417 | - |
L-homocysteine | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.417 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Candida albicans | |
0.533 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae | |
0.6 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | vitamin-B12 independent enzyme | Saccharomyces cerevisiae | |
additional information | vitamin-B12 independent enzyme | Candida albicans |