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Literature summary for 2.1.1.14 extracted from

  • Suliman, H.S.; Sawyer, G.M.; Appling, D.R.; Robertus, J.D.
    Purification and properties of cobalamin-independent methionine synthase from Candida albicans and Saccharomyces cerevisiae (2005), Arch. Biochem. Biophys., 441, 56-63.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain DAY4.1 Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain DAY4.1 Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.014
-
L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.084
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.094
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.109
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.113
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.12
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.129
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography Candida albicans
HiTrap heparin column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
29.3 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 5.6fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
additional information
-
42.11 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 11.7fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
r
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
r
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
-
Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
-
Saccharomyces cerevisiae tetrahydropteroyltri-L-glutamate + L-methionine
-
?
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
-
Candida albicans tetrahydropteroyltri-L-glutamate + L-methionine
-
?
additional information does not use 5-methyltetrahydropteroylglutamate as a substrate Saccharomyces cerevisiae ?
-
?
additional information does not use 5-methyltetrahydropteroylglutamate as a substrate Candida albicans ?
-
?

Synonyms

Synonyms Comment Organism
cobalamin-independent methionine synthase
-
Saccharomyces cerevisiae
cobalamin-independent methionine synthase
-
Candida albicans
Met6p
-
Saccharomyces cerevisiae
Met6p
-
Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.283
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.33
-
L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.35
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.4
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.417
-
L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.417
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
0.533
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
0.6
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information vitamin-B12 independent enzyme Saccharomyces cerevisiae
additional information vitamin-B12 independent enzyme Candida albicans