Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.136 extracted from

  • Coque, J.J.; Alvarez-Rodriguez, M.L.; Larriba, G.
    Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines (2003), Appl. Environ. Microbiol., 69, 5089-5095.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.136

Activating Compound

Activating Compound Comment Organism Structure
5-methyltetrahydrofolic acid 1 mM, activation to 119% of control Trichoderma longibrachiatum
DTT 1 mM + 1 mM PMSF, activation to 147% of control Trichoderma longibrachiatum
glycerol 10%, activation to 122% of control Trichoderma longibrachiatum
PMSF 1 mM + 1 mM DTT, activation to 147% of control Trichoderma longibrachiatum

General Stability

General Stability Organism
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity Trichoderma longibrachiatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 94% inhibition Trichoderma longibrachiatum
Ca2+ 1 mM, 16% inhibition Trichoderma longibrachiatum
Cu2+ 1 mM, 96% inhibition Trichoderma longibrachiatum
Hg2+ 0.1 mM, 96% inhibition Trichoderma longibrachiatum
S-adenosyl-L-homocysteine competitive Trichoderma longibrachiatum
Zn2+ 0.1 mM,82% inhibition Trichoderma longibrachiatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1359
-
 2,4,6-Trichlorophenol
-
 Trichoderma longibrachiatum
0.284
-
 S-adenosyl-L-methionine
-
 Trichoderma longibrachiatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
 2 * 52500, SDS-PAGE Trichoderma longibrachiatum
112000
-
 gel filtration Trichoderma longibrachiatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4,6-trichlorophenol + S-adenosyl-L-methionine Trichoderma longibrachiatum activity is induced by 2,4,6-trichlorophenol 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-trichlorophenol + S-adenosyl-L-methionine Trichoderma longibrachiatum CECT 20431 activity is induced by 2,4,6-trichlorophenol 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma longibrachiatum
-
 CECT 20431
-
Trichoderma longibrachiatum CECT 20431
-
 CECT 20431
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma longibrachiatum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trichoderma longibrachiatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00055
-
-
 Trichoderma longibrachiatum

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, stable for up to 3 months Trichoderma longibrachiatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine 10.7% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine 10.7% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3,4-trichlorophenol + S-adenosyl-L-methionine 93.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3,4-trichlorophenol + S-adenosyl-L-methionine 93.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3,6-trichlorophenol + S-adenosyl-L-methionine 55.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3,6-trichlorophenol + S-adenosyl-L-methionine 55.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,3-dichlorophenol + S-adenosyl-L-methionine 22.5% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,5-trichlorophenol + S-adenosyl-L-methionine 64.2% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-tribromophenol + S-adenosyl-L-methionine 13% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-trichlorophenol + S-adenosyl-L-methionine activity is induced by 2,4,6-trichlorophenol Trichoderma longibrachiatum 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-trichlorophenol + S-adenosyl-L-methionine 60% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-trichlorophenol + S-adenosyl-L-methionine activity is induced by 2,4,6-trichlorophenol Trichoderma longibrachiatum CECT 20431 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4,6-trichlorophenol + S-adenosyl-L-methionine 60% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum CECT 20431 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,4-dibromophenol + S-adenosyl-L-methionine 36.8% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
 ?
2,4-dichlorophenol + S-adenosyl-L-methionine
-
 Trichoderma longibrachiatum 2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,5-dichlorophenol + S-adenosyl-L-methionine 19.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2,6-dichlorophenol + S-adenosyl-L-methionine 10.4% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?
2-chlorophenol + S-adenosyl-L-methionine 9.6% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 2-chloroanisole + S-adenosyl-L-homocysteine
-
 ?
3,4-dichlorophenol + S-adenosyl-L-methionine 9.3% of the O-methylation activity with 2,3-dichlorophenol Trichoderma longibrachiatum 3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52500, SDS-PAGE Trichoderma longibrachiatum

Synonyms

Synonyms Comment Organism
CPOMT
-
 Trichoderma longibrachiatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Trichoderma longibrachiatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 54% loss of activity after 30 min, 89% loss of activity after 1 h Trichoderma longibrachiatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.5
-
 Trichoderma longibrachiatum

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum Trichoderma longibrachiatum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3789
-
 S-adenosyl-L-homocysteine
-
 Trichoderma longibrachiatum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma longibrachiatum chromatofocusing pH-range 4-7
-
 4.9