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Literature summary for 2.1.1.13 extracted from

  • Goulding, C.W.; Matthews, R.G.
    Cobalamin-dependent methionine synthase from Escherichia coli: Involvement of zinc in homocysteine activation (1997), Biochemistry, 36, 15749-15757.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Cys310Ser contains less than 0.05 equiv. of zinc, no proton release upon incubation with L-homocysteine, no enzymativc activity of holoenzyme Escherichia coli
Cys311Ser contains less than 0.05 equiv. of zinc, no proton release upon incubation with L-homocysteine, no enzymativc activity of holoenzyme Escherichia coli
additional information truncated MetH(2-649) protein, contains 0.9 equiv. of zinc, still proton release upon incubation with L-homocysteine Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 0.9 equiv. of zinc per mole, removal results in inactive enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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