Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using Tris, pH 8.0, 24% (w/v) PEG 6000 for enzyme without substrate, and 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 1.26 M ammonium sulfate for enzyme in complex with uroporphyrinogen III | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E114Q | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
G189K | the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
G189N | the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
H161F | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
K102A | the mutant shows no activity and strongly reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
M186L | the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
R111K | the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
R149K | the mutant shows no activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R51K | the mutant shows reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P95417 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas aeruginosa | 2 S-adenosyl-L-homocysteine + precorrin-2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
NirE | - |
Pseudomonas aeruginosa |
S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase | - |
Pseudomonas aeruginosa |