Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.107 extracted from

  • Woodcock, S.C.; Raux, E.; Levillayer, F.; Thermes, C.; Rambach, A.; Warren, M.J.
    Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of siroheme synthase (CysG) on siroheme and cobalamin biosynthesis (1998), Biochem. J., 330, 121-129.
No PubMed abstract available
Search for more literature for 2.1.1.107

Cloned(Commentary)

Cloned (Comment) Organism
complementation experiments with cysteine autotroph strain Escherichia coli

Protein Variants

Protein Variants Comment Organism
D227A full activity Escherichia coli
D248A no transmethylase activity Escherichia coli
D303A full activity Escherichia coli
G224A unable to bind S-adenosyl-L-methionine Escherichia coli
K270I full activity Escherichia coli
R298L unable to bind S-adenosyl-L-methionine Escherichia coli
R309L no transmethylase activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III Escherichia coli involved in biosynthesis of cobalamin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III methylates positions 2 and 7 Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of cobalamin Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?