Cloned (Comment) | Organism |
---|---|
cysG containing plasmid transformed into Escherichia coli CBK103 | Escherichia coli |
overexpression of cysG gene | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49930 | - |
calculated from DNA-sequence | Escherichia coli |
52000 | - |
SDS-PAGE | Escherichia coli |
66000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | Escherichia coli | involved in biosynthesis of sirohydrochlorin | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | methylates positions 2 and 7 | Escherichia coli | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of sirohydrochlorin | Escherichia coli | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE, gel filtration | Escherichia coli |