Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Vitis vinifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Vitis vinifera | |
Mn2+ | - |
Vitis vinifera | |
Zn2+ | - |
Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0827 | - |
cyanidin 3-O-glucoside | thrombin-cleaved enzyme, at pH 8.5 and 30°C | Vitis vinifera | |
0.096 | - |
caffeoyl-CoA | GST-tagged recombinant enzyme, at pH 8.5 and 30°C | Vitis vinifera | |
0.11 | - |
cyanidin 3-O-glucoside | GST-tagged recombinant enzyme, at pH 8.5 and 30°C | Vitis vinifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM used in assay conditions | Vitis vinifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | Q43237 | cultivar Barbera | - |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Vitis vinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Vitis vinifera | - |
leaf | - |
Vitis vinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Vitis vinifera | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + cyanidin 3-O-glucoside | - |
Vitis vinifera | S-adenosyl-L-homocysteine + peonidin 3-O-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCoAOMT | - |
Vitis vinifera |
COMT | - |
Vitis vinifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
cyanidin 3-O-glucoside | GST-tagged recombinant enzyme, at pH 8.5 and 30°C | Vitis vinifera | |
0.0082 | - |
caffeoyl-CoA | GST-tagged recombinant enzyme, at pH 8.5 and 30°C | Vitis vinifera | |
0.0129 | - |
cyanidin 3-O-glucoside | thrombin-cleaved enzyme, at pH 8.5 and 30°C | Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
optimal pH value for the methylation of cyanidin 3-O-glucoside | Vitis vinifera |
Organism | Comment | Expression |
---|---|---|
Vitis vinifera | transcript accumulation of the enzyme is detected in berry skins, and increases during berry ripening on the plant, and in cultured berries treated with abscisic acid. Drought stress increases the enzyme transcript in berries | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme contributes to anthocyanin methylation activity in grape berries, in particular under drought stress | Vitis vinifera |