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Literature summary for 2.1.1.104 extracted from

  • Do, C.T.; Pollet, B.; Thevenin, J.; Sibout, R.; Denoue, D.; Barriere, Y.; Lapierre, C.; Jouanin, L.
    Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in Arabidopsis (2007), Planta, 226, 1117-1129.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, pGEM-T easy vector, pCAMBIA1391xb vector, transgenic plants produced, promoter-GUS fusion constructs, T-DNA null mutant of GABI T-DNA insertion collection identified and characterized, double mutants lacking caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1) Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA Arabidopsis thaliana enzyme predicted to catalyze first methylation step during biosynthesis of monolignols S-adenosyl-L-homocysteine + feruloyl-CoA
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?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49499 At4g34050; ecotypes Columbia (Col-0) and Wassilewskija (WS)
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Purification (Commentary)

Purification (Comment) Organism
mutant and wild-type protein Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Arabidopsis thaliana
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stem
-
Arabidopsis thaliana
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vascular tissue interfascicular fibres Arabidopsis thaliana
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xylem
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Arabidopsis thaliana
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
T-DNA null mutant indicates collapsed xylem elements under short-day conditions, lignin composition of p-hydroxyphenyl units and inability of sinapoyl malate synthesis in double mutants impaired in caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1), C3 methylation of the phenolic ring of monolignols in Arabidopsis mediated by both, caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1), both enzymes also involved in formation of sinapoyl malate and isorhamnetin Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA enzyme predicted to catalyze first methylation step during biosynthesis of monolignols Arabidopsis thaliana S-adenosyl-L-homocysteine + feruloyl-CoA
-
?
S-adenosyl-L-methionine + caffeoyl-CoA enzyme responsible for the first methylation step during biosynthesis of monolignols Arabidopsis thaliana S-adenosyl-L-homocysteine + feruloyl-CoA
-
?

Synonyms

Synonyms Comment Organism
CCoAOMT
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Arabidopsis thaliana