Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ammi majus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.15 mM, completely abolishes the rate of methylation of caffeoyl-CoA | Ammi majus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
caffeoyl-CoA | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.009 | - |
quercetagetin | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.02 | - |
caffeoyl-CoA | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.031 | - |
quercetin | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.039 | - |
3,4-dihydroxybenzoic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.061 | - |
caffeoyl-D-glucose | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.068 | - |
5-hydroxyferulic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.073 | - |
quercetin | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.088 | - |
caffeic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.93 | - |
caffeoyl-D-glucose | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Co2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor | Ammi majus | |
Mg2+ | required | Ammi majus | |
Mn2+ | 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Mn2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor | Ammi majus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Ammi majus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ammi majus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Ammi majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:dark-grown cell | - |
Ammi majus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid | activity in presence of Co2+, no activity in presence of Mg2+ | Ammi majus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-ferulic acid | activity in presence of Co2+, no activity in presence of Mg2+ | Ammi majus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeic acid | activity in presence of Co2+, no activity in presence of Mg2+ | Ammi majus | S-adenosyl-L-homocysteine + ferulic acid | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Ammi majus | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-D-glucose | - |
Ammi majus | S-adenosyl-L-homocysteine + feruloyl-D-glucose | - |
? | |
S-adenosyl-L-methionine + quercetagetin | no activity in presence of Co2+, low activity in presence of Mg2+ | Ammi majus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Ammi majus | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Ammi majus |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl-coenzyme A O-methyltransferase | - |
Ammi majus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
3,4-dihydroxybenzoic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.068 | - |
caffeic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.071 | - |
quercetagetin | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.143 | - |
5-hydroxyferulic acid | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.149 | - |
caffeoyl-CoA | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.206 | - |
caffeoyl-D-glucose | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.268 | - |
quercetin | pH 7.5, 37°C, in presence of 0.15 mM Mg2+ | Ammi majus | |
0.631 | - |
caffeoyl-D-glucose | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
0.938 | - |
quercetin | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
1.043 | - |
caffeoyl-CoA | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
6.08 | - |
quercetin | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
6.08 | - |
caffeoyl-CoA | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus | |
6.08 | - |
caffeoyl-D-glucose | pH 7.5, 37°C, in presence of 0.1 mM Co2+ | Ammi majus |