Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
D128A mutant in complex with S-adenosylhomocysteine and either phosphoethanolamine or phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 8000, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl)phosphine, and 5 mM S-adenosyl-L-homocysteine | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
D128A | compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 91% activity) | Plasmodium falciparum |
D128E | compared to the wild type enzyme, the mutant shows about 90% loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 182% activity) | Plasmodium falciparum |
D128N | compared to the wild type enzyme, the mutant shows complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 24% activity) | Plasmodium falciparum |
D128Q | compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 89% activity) | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
Ethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.181 | - |
N,N-dimethylethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.773 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.786 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
7.99 | - |
Ethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
21.17 | - |
Ethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate | Plasmodium falciparum | - |
S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q6T755 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity column chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate | - |
Plasmodium falciparum | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + N,N-dimethylethanolamine phosphate | - |
Plasmodium falciparum | S-adenosyl-L-homocysteine + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphoethanolamine methyltransferase | - |
Plasmodium falciparum |
PMT | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Ethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.022 | - |
Ethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.367 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum | |
1.483 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
1.82 | - |
Ethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum | |
3.08 | - |
N,N-dimethylethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is essential for normal growth and development | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
Ethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.00271 | - |
Ethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
0.474 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128N, at pH 8.0 and 20°C | Plasmodium falciparum | |
1.887 | - |
N,N-dimethylethanolamine phosphate | mutant enzyme D128A, at pH 8.0 and 20°C | Plasmodium falciparum | |
17.04 | - |
N,N-dimethylethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum | |
33.64 | - |
Ethanolamine phosphate | wild type enzyme, at pH 8.0 and 20°C | Plasmodium falciparum |