Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential target for development of inhibitors | Caenorhabditis elegans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caenorhabditis elegans |
gene pmt-1, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain Rosetta II (DE3) | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene pmt-1 knockout by RNAi, exposure to arsenite and phenotype in different developmental stages, overview | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-methylethanolamine phosphate | competitive inhibition versus both substrates | Caenorhabditis elegans | |
phosphocholine | competitive feedback inhibition; feedback inhibition | Caenorhabditis elegans | |
S-adenosyl-L-homocysteine | competitive versus S-adenosyl-L-methionine, non-competitive versus ethanolamine phosphate; product inhibition versus SAM and phosphoethanolamine show competitive and non-competitive inhibition patterns, respectively | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | PMT-1 uses a random sequential kinetic mechanism | Caenorhabditis elegans | |
0.0099 | - |
Ethanolamine phosphate | - |
Caenorhabditis elegans | |
0.145 | - |
S-adenosyl-L-methionine | - |
Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
1 * 52000, SDS-PAGE | Caenorhabditis elegans |
55100 | - |
calculated from sequence | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate | Caenorhabditis elegans | first reaction of a pathway for phosphocholine biosynthesis. PMT-1 functions at a step before monomethylethanolamine enters the plant-like phosphobase methylation pathway | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + phosphoethanolamine | Caenorhabditis elegans | first step in the PEAMTpathway, i.e. S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferases pathway, the enzyme pmt-1 is required for worm growth and development | S-adenosyl-L-homocysteine + methylethanolamine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Caenorhabditis elegans | - |
gene pmt-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Caenorhabditis elegans |
recombinant His6-tagged PMT from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine adsorption chromatography, and gel filtration | Caenorhabditis elegans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | random sequential kinetic mechanism | Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
- |
Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate | first reaction of a pathway for phosphocholine biosynthesis. PMT-1 functions at a step before monomethylethanolamine enters the plant-like phosphobase methylation pathway | Caenorhabditis elegans | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + phosphoethanolamine | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + phosphoethanolamine | first step in the PEAMTpathway, i.e. S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferases pathway, the enzyme pmt-1 is required for worm growth and development | Caenorhabditis elegans | S-adenosyl-L-homocysteine + methylethanolamine phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
PEAMT | - |
Caenorhabditis elegans |
phosphoethanolamine N-methyltransferase | - |
Caenorhabditis elegans |
PMT-1 | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
Ethanolamine phosphate | - |
Caenorhabditis elegans | |
0.058 | - |
S-adenosyl-L-methionine | - |
Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Caenorhabditis elegans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | product inhibition patterns are indicative of a random sequential Bi Bi kinetic mechanism | Caenorhabditis elegans | |
0.0048 | - |
S-adenosyl-L-homocysteine | 30°C, pH 8, varied substrate: ethanolamine phosphate | Caenorhabditis elegans | |
0.0091 | - |
S-adenosyl-L-homocysteine | 30°C, pH 8, varied substrate: S-adenosyl-L-methionine | Caenorhabditis elegans | |
4.23 | - |
N-methylethanolamine phosphate | 30°C, pH 8, varied substrate: ethanolamine phosphate | Caenorhabditis elegans | |
5 | - |
phosphocholine | about, pH 8.0, 30°C, recombinant enzyme | Caenorhabditis elegans | |
5.11 | - |
N-methylethanolamine phosphate | 30°C, pH 8, varied substrate: S-adenosyl-L-methionine | Caenorhabditis elegans | |
6.94 | - |
phosphocholine | 30°C, pH 8, varied substrate: ethanolamine phosphate | Caenorhabditis elegans | |
7.81 | - |
phosphocholine | 30°C, pH 8, varied substrate: S-adenosyl-L-methionine | Caenorhabditis elegans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Caenorhabditis elegans | calculated from sequence | - |
5.1 |