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Literature summary for 2.1.1.103 extracted from

  • Brendza, K.M.; Haakenson, W.; Cahoon, R.E.; Hicks, L.M.; Palavalli, L.H.; Chiapelli, B.J.; McLaird, M.; McCarter, J.P.; Williams, D.J.; Hresko, M.C.; Jez, J.M.
    Phosphoethanolamine N-methyltransferase (PMT-1) catalyzes the first reaction of a new pathway for phosphocholine biosynthesis in Caenorhabditis elegans (2007), Biochem. J., 404, 439-448.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.103

Application

Application Comment Organism
drug development the enzyme is a potential target for development of inhibitors Caenorhabditis elegans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caenorhabditis elegans
gene pmt-1, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain Rosetta II (DE3) Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
additional information gene pmt-1 knockout by RNAi, exposure to arsenite and phenotype in different developmental stages, overview Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
N-methylethanolamine phosphate competitive inhibition versus both substrates Caenorhabditis elegans
phosphocholine competitive feedback inhibition; feedback inhibition Caenorhabditis elegans
S-adenosyl-L-homocysteine competitive versus S-adenosyl-L-methionine, non-competitive versus ethanolamine phosphate; product inhibition versus SAM and phosphoethanolamine show competitive and non-competitive inhibition patterns, respectively Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information PMT-1 uses a random sequential kinetic mechanism Caenorhabditis elegans
0.0099
-
 Ethanolamine phosphate
-
 Caenorhabditis elegans
0.145
-
 S-adenosyl-L-methionine
-
 Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 1 * 52000, SDS-PAGE Caenorhabditis elegans
55100
-
 calculated from sequence Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + ethanolamine phosphate Caenorhabditis elegans first reaction of a pathway for phosphocholine biosynthesis. PMT-1 functions at a step before monomethylethanolamine enters the plant-like phosphobase methylation pathway S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine Caenorhabditis elegans first step in the PEAMTpathway, i.e. S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferases pathway, the enzyme pmt-1 is required for worm growth and development S-adenosyl-L-homocysteine + methylethanolamine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Caenorhabditis elegans
-
 gene pmt-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Caenorhabditis elegans
recombinant His6-tagged PMT from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine adsorption chromatography, and gel filtration Caenorhabditis elegans

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate random sequential kinetic mechanism Caenorhabditis elegans
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + ethanolamine phosphate first reaction of a pathway for phosphocholine biosynthesis. PMT-1 functions at a step before monomethylethanolamine enters the plant-like phosphobase methylation pathway Caenorhabditis elegans S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine first step in the PEAMTpathway, i.e. S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferases pathway, the enzyme pmt-1 is required for worm growth and development Caenorhabditis elegans S-adenosyl-L-homocysteine + methylethanolamine phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
PEAMT
-
 Caenorhabditis elegans
phosphoethanolamine N-methyltransferase
-
 Caenorhabditis elegans
PMT-1
-
 Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052
-
 Ethanolamine phosphate
-
 Caenorhabditis elegans
0.058
-
 S-adenosyl-L-methionine
-
 Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Caenorhabditis elegans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information product inhibition patterns are indicative of a random sequential Bi Bi kinetic mechanism Caenorhabditis elegans
0.0048
-
 S-adenosyl-L-homocysteine 30°C, pH 8, varied substrate: ethanolamine phosphate Caenorhabditis elegans
0.0091
-
 S-adenosyl-L-homocysteine 30°C, pH 8, varied substrate: S-adenosyl-L-methionine Caenorhabditis elegans
4.23
-
 N-methylethanolamine phosphate 30°C, pH 8, varied substrate: ethanolamine phosphate Caenorhabditis elegans
5
-
 phosphocholine about, pH 8.0, 30°C, recombinant enzyme Caenorhabditis elegans
5.11
-
 N-methylethanolamine phosphate 30°C, pH 8, varied substrate: S-adenosyl-L-methionine Caenorhabditis elegans
6.94
-
 phosphocholine 30°C, pH 8, varied substrate: ethanolamine phosphate Caenorhabditis elegans
7.81
-
 phosphocholine 30°C, pH 8, varied substrate: S-adenosyl-L-methionine Caenorhabditis elegans

pI Value

Organism Comment pI Value Maximum pI Value
Caenorhabditis elegans calculated from sequence
-
 5.1