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Literature summary for 2.1.1.101 extracted from

  • Yeh, W.K.; Bauer, N.J.; Dotzlaf, J.E.
    High-performance liquid chromatographic assay for S-adenosyl-L-methionine: macrocin O-methyltransferase (1984), J. Chromatogr., 288, 157-165.
    View publication on PubMed
Search for more literature for 2.1.1.101

Inhibitors

Inhibitors Comment Organism Structure
Relomycin
-
 Streptomyces fradiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 S-adenosyl-L-methionine
-
 Streptomyces fradiae
0.033
-
 macrocin
-
 Streptomyces fradiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulated by cobalt chloride Streptomyces fradiae
Fe2+ stimulated by iron (II) sulfate Streptomyces fradiae
Mg2+ stimulated by magnesium sulfate Streptomyces fradiae
Mn2+ stimulated by manganese sulfate Streptomyces fradiae
Zn2+ stimulated by zinc sulfate Streptomyces fradiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + macrocin Streptomyces fradiae terminal and main rate-limiting step in tylosin biosynthesis S-adenosylhomocysteine + tylosin
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Storage Stability

Storage Stability Organism
4°C, enzyme is unstable, about 30% activity remains after 3 h Streptomyces fradiae
4°C, very stable for 3 h in presence of 10% ethanol Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + macrocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + tylosin
-
 ?
S-adenosyl-L-methionine + macrocin terminal and main rate-limiting step in tylosin biosynthesis Streptomyces fradiae S-adenosylhomocysteine + tylosin
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Streptomyces fradiae