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Literature summary for 2.1.1.100 extracted from
- Functional oligomerization of the Saccharomyces cerevisiae isoprenylcysteine carboxyl methyltransferase, Ste14p (2010), J. Biol. Chem., 285, 13380-13387.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain SM1188 | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E213Q | the mutant is enzymatically almost inactive (5% activity compared to the wild type) | Saccharomyces cerevisiae |
| L81F | the mutant shows little to no in vitro methyltransferase activity | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
| membrane | an integral membrane protein | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, untagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
| 35000 | - |
2 * 35000, His-tagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity bead chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.755 | - |
wild type enzyme, at pH and °C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 23000, untagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
| homodimer | 2 * 35000, His-tagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Icmt | - |
Saccharomyces cerevisiae |
| isoprenylcysteine carboxyl methyltransferase | - |
Saccharomyces cerevisiae |
| Ste14p | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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