Any feedback?
Literature summary for 2.1.1.10 extracted from
- A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation (1999), J. Biol. Chem., 274, 5407-5414.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-methionine | product inhibition above 0.5 mM | Escherichia coli |  |
| S-adenosyl-L-methionine | product inhibition above 0.25 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
L-homocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32500 | - |
1 * 32500, calculated from sequence | Escherichia coli |
| 34000 | - |
gel filtration | Escherichia coli |
| 35000 | - |
1 * 35000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-L-methionine + L-homocysteine | Escherichia coli | - |
2 L-methionine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q47690 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also catalyzes the reaction of selenocysteine methyltransferase | Escherichia coli | ? | - |
? | |
| S-adenosyl-L-methionine + L-homocysteine | - |
Escherichia coli | S-adenosyl-L-homocysteine + L-methionine | - |
? | |
| S-methyl-L-methionine + L-homocysteine | - |
Escherichia coli | 2 L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Escherichia coli |
| monomer | 1 * 32500, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YagD | - |
Escherichia coli |
| YagD protein | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
L-homocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
