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Literature summary for 2.1.1.1 extracted from

  • Alston, T.A.; Abeles, R.H.
    Substrate specificity of nicotinamide methyltransferase isolated from porcine liver (1988), Arch. Biochem. Biophys., 260, 601-608.
    View publication on PubMed
Search for more literature for 2.1.1.1

General Stability

General Stability Organism
glycerol stabilizes Sus scrofa
thiols required for stability during isolation Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 S-adenosyl-L-methionine
-
 Sus scrofa
0.2
-
 1,2,3,4-tetrahydroisoquinoline
-
 Sus scrofa
0.83
-
 nicotinamide
-
 Sus scrofa
1.3
-
 Quinoline
-
 Sus scrofa
1.5
-
 Isoquinoline
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 1 * 27000, SDS-PAGE Sus scrofa
27000 30000 gel filtration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa enzyme may function in detoxificating of numerous alkaloids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol w/v, stable for 1 month, partially purified enzyme Sus scrofa
4°C, 20% glycerol w/v, pH 7.6, 30% loss of activity, purified enzyme Sus scrofa
4°C, 20% w/v glycerol, stable for 1 week, partially purified enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme may function in detoxificating of numerous alkaloids Sus scrofa ?
-
 ?
S-adenosyl-L-methionine + 1,2,3,4-tetrahydroisoquinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + 3-acetylpyridine
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + isoquinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + nicotinamide
-
 Sus scrofa S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
S-adenosyl-L-methionine + quinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + thionicotinamide
-
 Sus scrofa S-adenosyl-L-homocysteine + 1-methylthionicotinamide
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.7 9.8 about 50% of maximum activity at pH 5.7 and 9.8 Sus scrofa