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Literature summary for 1.97.1.9 extracted from
- Thermostable properties of the periplasmic selenate reductase from Thauera selenatis (2010), Biochimie, 92, 1268-1273.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tungstate | SER isolated from periplasmic fractions from cells grown on 1 mM tungstate display selenate reductase activities with a 20fold reduction in Vmax and a 23fold increase in substrate binding affinity. The thermo-stability and pH dependence of tungsten-SER is shown to be similar to that observed for molybdenum-SER | Thauera selenatis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
selenate | tungsten-SER, pH and temperature not specified in the publication, Vmax: 0.01 micromol/min/mg | Thauera selenatis | |
| 0.016 | - |
selenate | molybdenum-SER, pH and temperature not specified in the publication, Vmax: 0.2 micromol/min/mg | Thauera selenatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | - |
Thauera selenatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
| 40000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
| 96000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera selenatis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| selenate + reduced methyl viologen | - |
Thauera selenatis | selenite + H2O + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| selenate reductase | - |
Thauera selenatis |
| SER | - |
Thauera selenatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
optimal temperature for molybdenum-SER | Thauera selenatis |
| 80 | - |
optimal temperature for tungsten-SER is higher than 80°C | Thauera selenatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified molybdenum-SER complex is stable and active upon heat-shock incubation for 10 min at temperatures up to 60°C. At temperatures greater than 65°C all three subunits (SerABC) are readily denatured | Thauera selenatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thauera selenatis |
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