Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.97.1.9 extracted from

  • Ridley, H.; Watts, C.A.; Richardson, D.J.; Butler, C.S.
    Resolution of distinct membrane-bound enzymes from Enterobacter cloacae SLD1a-1 that are responsible for selective reduction of nitrate and selenate oxyanions (2006), Appl. Environ. Microbiol., 72, 5173-5180.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.97.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 selenate pH 7.2, 30°C, holenzyme complex Enterobacter cloacae
5.5
-
 selenate pH 7.2, 30°C, isolated alpha subunit Enterobacter cloacae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Enterobacter cloacae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme iron, 18 mol per mol of enzyme Enterobacter cloacae
Molybdenum 0.6 mol per mol of enzyme Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE Enterobacter cloacae
55000
-
 3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE Enterobacter cloacae
600000
-
 gel filtration Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 expression under aerobic conditions, poor expression during anaerobic growth on nitrate
-
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
 expression under aerobic conditions, poor expression during anaerobic growth on nitrate
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
500
-
 pH 7.2, 30°C Enterobacter cloacae

Storage Stability

Storage Stability Organism
-20°C, enzyme extracted with Thesit retains 50% activity after being frozen for prolonged periods Enterobacter cloacae
4°C, enzyme extracted with Thesit remains active for 24 h Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: nitrate, sulfate, perchlorate, thiosulfate Enterobacter cloacae ?
-
 ?
additional information no substrate: nitrate, sulfate, perchlorate, thiosulfate Enterobacter cloacae SLD1a-1 / ATCC 700258 ?
-
 ?
selenate + electron donor
-
 Enterobacter cloacae selenite + H2O + oxidized donor
-
 ?
selenate + electron donor
-
 Enterobacter cloacae SLD1a-1 / ATCC 700258 selenite + H2O + oxidized donor
-
 ?

Subunits

Subunits Comment Organism
nonamer 3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE Enterobacter cloacae

Cofactor

Cofactor Comment Organism Structure
heme 0.9 mol per mol of enzyme Enterobacter cloacae