Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by sodium azide | Enterobacter cloacae | |
tungstate | in vitro and in vivo | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.25 | - |
benzyl viologen | 20°C | Enterobacter cloacae | |
6.25 | - |
selenate | with reduced benzyl viologen, 20°C | Enterobacter cloacae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | with localisation of the catalytic site to the periplasmic side of the membrane | Enterobacter cloacae | - |
- |
membrane | - |
Enterobacter cloacae | 16020 | - |
additional information | not in the cytosol | Enterobacter cloacae | - |
- |
periplasm | - |
Enterobacter cloacae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | required | Enterobacter cloacae | |
Molybdenum | enhances the activity 10fold in vivo when added to the growth medium | Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + reduced acceptor | Enterobacter cloacae | detoxification of selenate | selenite + H2O + acceptor | - |
? | |
selenate + reduced acceptor | Enterobacter cloacae SLD1a-1 / ATCC 700258 | detoxification of selenate | selenite + H2O + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Enterobacter cloacae | - |
highest activity when molybdate is added to the culture medium | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | - |
- |
- |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | - |
highest activity when molybdate is added to the culture medium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.29 | - |
membrane fraction | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + electron donor | - |
Enterobacter cloacae | selenite + H2O + oxidized electron donor | - |
? | |
selenate + electron donor | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + oxidized electron donor | - |
? | |
selenate + reduced acceptor | detoxification of selenate | Enterobacter cloacae | selenite + H2O + acceptor | - |
? | |
selenate + reduced acceptor | detoxification of selenate | Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + acceptor | - |
? | |
selenate + reduced benzyl viologen | - |
Enterobacter cloacae | selenite + H2O + oxidized benzyl viologen | - |
? | |
selenate + reduced benzyl viologen | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + oxidized benzyl viologen | - |
? | |
selenate + reduced benzyl viologen | - |
Enterobacter cloacae | selenite + H2O + benzyl viologen | - |
? | |
selenate + reduced benzyl viologen | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + benzyl viologen | - |
? | |
selenate + reduced methyl viologen | preferred electron donor | Enterobacter cloacae | selenite + H2O + oxidized methyl viologen | - |
? | |
selenate + reduced methyl viologen | preferred electron donor | Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + oxidized methyl viologen | - |
? | |
selenate + reduced methyl viologen | - |
Enterobacter cloacae | selenite + H2O + methyl viologen | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Enterobacter cloacae |