KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
selenate | pH 6.0 | Thauera selenatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Thauera selenatis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | part of at least 2 iron-sulfur centres per enzyme molecule and of the heme group of cytochrome b | Thauera selenatis | |
Iron | 12.9 mol per mol of enzyme, based on MW 159000 | Thauera selenatis | |
Molybdenum | 1 molybdenum per mol of trimer | Thauera selenatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE | Thauera selenatis |
40000 | - |
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE | Thauera selenatis |
96000 | - |
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE | Thauera selenatis |
180000 | - |
gel filtration | Thauera selenatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + electron donor | Thauera selenatis | selenate is the terminal electron acceptor in dissimilatory selenate reduction | selenite + H2O + oxidized electron donor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera selenatis | - |
selenate-respiring bacterium | - |
Purification (Comment) | Organism |
---|---|
57fold, to near homogeneity | Thauera selenatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.4 | - |
purified enzyme | Thauera selenatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NADH, succinate, and lactate are no electron donors | Thauera selenatis | ? | - |
? | |
additional information | no activity with nitrate, nitrite, sulfate, and chlorate | Thauera selenatis | ? | - |
? | |
selenate + electron donor | specific for selenate | Thauera selenatis | selenite + H2O + oxidized electron donor | - |
? | |
selenate + electron donor | selenate is the terminal electron acceptor in dissimilatory selenate reduction | Thauera selenatis | selenite + H2O + oxidized electron donor | - |
? | |
selenate + reduced benzyl viologen | best electron donor | Thauera selenatis | selenite + H2O + oxidized benzyl viologen | - |
? | |
selenate + reduced methyl viologen | 11% of the activity with benzyl viologen | Thauera selenatis | selenite + H2O + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 1 cytochrome b per alphabetagamma-complex | Thauera selenatis |
trimer | 1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE | Thauera selenatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
387 | - |
selenate | pH 6.0 | Thauera selenatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thauera selenatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | 1 cytochrome b per alphabetagamma-trimer | Thauera selenatis | |
iron-sulfur centre | at least 2 [Fe-S]-centre as prosthetic groups per enzyme molecule | Thauera selenatis |