Cloned (Comment) | Organism |
---|---|
cloning of the luminal domain of spinach photosystem 1 subunit PsaF, expressed in Escherichia coli BL21 (DE3) using a pET32 Xa/LIC thioredoxin fusion system | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced plastocyanin + oxidized ferredoxin + hv | Spinacia oleracea | - |
oxidized plastocyanin + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the luminal domain of spinach photosystem 1 subunit PsaF | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced plastocyanin + oxidized ferredoxin + hv | - |
Spinacia oleracea | oxidized plastocyanin + reduced ferredoxin | - |
? | |
reduced plastocyanin + oxidized ferredoxin + hv | the photosystem 1 subunit PsaF is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, the recombinant protein binds to plastocyanin by a specific, native-like, electrostatic interaction | Spinacia oleracea | oxidized plastocyanin + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 21000-22000 Da, photosystem 1 subunit PsaF that is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, SDS-PAGE | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
PsaF | - |
Spinacia oleracea |