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Literature summary for 1.97.1.1 extracted from

  • Martínez-Espinosa, R.M.; Richardson, D.J.; Bonete, M.J.
    Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei (2015), Biochim. Biophys. Acta, 1850, 587-594.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
Chlorate pH 8, 40°C Haloferax mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35 °C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-
Haloferax mediterranei ATCC 33500 I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorate + reduced methyl viologen
-
Haloferax mediterranei chlorite + H2O + methyl viologen
-
?
chlorate + reduced methyl viologen
-
Haloferax mediterranei ATCC 33500 chlorite + H2O + methyl viologen
-
?

Synonyms

Synonyms Comment Organism
NarGH
-
Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35°C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei
40 45 the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35°C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei
40
-
assay at Haloferax mediterranei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
Chlorate pH 8, 40°C Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Haloferax mediterranei

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.0: about 85% of maximal activity, pH 9.0: about 75% of maximal activity Haloferax mediterranei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
Chlorate pH 8, 40°C Haloferax mediterranei