Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | mixed type inhibition | Paracoccus pantotrophus | |
thiocyanate | competitive | Paracoccus pantotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
nitrate | pH 7, NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
x * 16000 + x * 90000, SDS-PAGE | Paracoccus pantotrophus |
90000 | - |
x * 16000 + x * 90000, SDS-PAGE | Paracoccus pantotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry. Exploration of the nitrate reductase activity of purified NapAB as a function of electrochemical potential, substrate concentration and pH using protein film voltammetry. Nitrate reduction by NapAB occurs at potentials below approx. 0.1 V at pH 7. These are lower potentials than required for NarGH nitrate reduction. The potentials required for Nap nitrate reduction are also likely to require ubiquinol/ubiquinone ratios higher than are needed to activate the H+-pumping oxidases expressed during aerobic growth where Nap levels are maximal. Thus the operational potentials of Paracoccus pantotrophus NapAB are consistent with a productive role in redox balancing | Paracoccus pantotrophus | ? | - |
? | |
nitrate + reduced methyl viologen | - |
Paracoccus pantotrophus | nitrite + oxidized methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16000 + x * 90000, SDS-PAGE | Paracoccus pantotrophus |
Synonyms | Comment | Organism |
---|---|---|
NapAB | - |
Paracoccus pantotrophus |