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Literature summary for 1.8.99.5 extracted from

  • Wolfe, B.M.; Lui, S.M.; Cowan, J.A.
    Desulfoviridin, a multimeric-dissimilatory sulfite reductase from Desulfovibrio vulgaris (Hildenborough). Purification, characterization, kinetics and EPR studies (1994), Eur. J. Biochem., 223, 79-89.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 nitrite pH 7.6, 22°C Desulfovibrio vulgaris
0.06
-
 sulfite pH 7.6, 22°C Desulfovibrio vulgaris
48
-
 hydroxylamine pH 7.6, 22°C Desulfovibrio vulgaris

Organism

Organism UniProt Comment Textmining
Desulfovibrio vulgaris
-
-
-
Desulfovibrio vulgaris Hildenborough
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
 Desulfovibrio vulgaris ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
-
 ?
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
 Desulfovibrio vulgaris Hildenborough ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
-
 ?
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
 Desulfovibrio vulgaris ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
-
 ?
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
 Desulfovibrio vulgaris Hildenborough ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
-
 ?
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
 Desulfovibrio vulgaris hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O overall reaction ?
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
 Desulfovibrio vulgaris Hildenborough hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O overall reaction ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 nitrite pH 7.6, 22°C Desulfovibrio vulgaris
0.31
-
 sulfite pH 7.6, 22°C Desulfovibrio vulgaris
29
-
 hydroxylamine pH 7.6, 22°C Desulfovibrio vulgaris

Cofactor

Cofactor Comment Organism Structure
siroheme at least two of three subunits contain two pairs of [Fe4-S4]-centers and siroheme units Desulfovibrio vulgaris
[4Fe-4S]-center at least two of three subunits contain two pairs of [Fe4-S4]-centers and siroheme units Desulfovibrio vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 hydroxylamine pH 7.6, 22°C Desulfovibrio vulgaris
1.4
-
 nitrite pH 7.6, 22°C Desulfovibrio vulgaris
5.17
-
 sulfite pH 7.6, 22°C Desulfovibrio vulgaris