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Literature summary for 1.8.99.2 extracted from

  • Kim, S.K.; Gomes, V.; Gao, Y.; Chandramouli, K.; Johnson, M.K.; Knaff, D.B.; Leustek, T.
    The two-domain structure of 5-adenylylsulfate (APS) reductase from Enteromorpha intestinalis is a requirement for efficient APS reductase activity (2007), Biochemistry, 46, 591-601.
    View publication on PubMed
Search for more literature for 1.8.99.2

Activating Compound

Activating Compound Comment Organism Structure
Na2SO4 500 mM, 5 fold stimulation Ulva intestinalis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ulva intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 dithiothreitol
-
 Ulva intestinalis
0.011
-
 5'-adenylylsulfate
-
 Ulva intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 calculated from sequence of cDNA, C-terminal domain of EiAPR Ulva intestinalis
20000
-
 MALDI-TOF mass spectrometry, C-terminal domain of EiAPR Ulva intestinalis
48700
-
 MALDI-TOF mass spectrometry, whole enzyme Ulva intestinalis
48710
-
 calculated from sequence of cDNA, whole enzyme Ulva intestinalis

Organism

Organism UniProt Comment Textmining
Ulva intestinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography Ulva intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylylsulfate + dithiothreitol
-
 Ulva intestinalis adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
 ?

Synonyms

Synonyms Comment Organism
5-adenylylsulfate reductase
-
 Ulva intestinalis
EiAPR
-
 Ulva intestinalis

pI Value

Organism Comment pI Value Maximum pI Value
Ulva intestinalis
-
-
 6.91