Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | 10 mM, complete inhibition | Thiobacillus denitrificans | |
NEM | 5 mM complete inhibition | Thiobacillus thioparus | |
PCMB | 0.1 mM, 30% inhibition | Thiobacillus denitrificans | |
PCMB | 1 mM, complete inhibition | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
AMP | reaction with cytochrome c | Thiobacillus thioparus | |
0.01 | - |
GMP | reaction with cytochrome c | Thiobacillus thioparus | |
0.017 | - |
sulfite | reaction with cytochrome c | Thiobacillus thioparus | |
0.041 | - |
AMP | reaction with ferricyanide | Thiobacillus denitrificans | |
0.1 | - |
AMP | reaction with ferricyanide | Thiobacillus thioparus | |
0.63 | - |
GMP | reaction with ferricyanide | Thiobacillus denitrificans | |
1.5 | - |
sulfite | reaction with ferricyanide | Thiobacillus denitrificans | |
2.5 | - |
sulfite | reaction with ferricyanide | Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 8-10 mol of non-heme iron per mol of enzyme | Thiobacillus thioparus | |
Iron | contains 8-13 mol of non-heme iron per mol of enzyme | Thiobacillus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus denitrificans | - |
- |
- |
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thiobacillus thioparus |
- |
Thiobacillus denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
5°C, 5 days, 20% loss of activity | Thiobacillus thioparus |
frozen, protein concentration above 5 mg/ml, stable for several months | Thiobacillus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + sulfite + cytochrome c | - |
Thiobacillus thioparus | adenylyl sulfate + ? | - |
? | |
AMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | adenylyl sulfate + ferrocyanide | - |
? | |
AMP + sulfite + ferricyanide | - |
Thiobacillus denitrificans | adenylyl sulfate + ferrocyanide | - |
? | |
CMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | cytidylyl sulfate + ferrocyanide | - |
? | |
CMP + sulfite + ferricyanide | no activity with CMP | Thiobacillus denitrificans | cytidylyl sulfate + ferrocyanide | - |
? | |
deoxyAMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | deoxyadenylyl sulfate + ferrocyanide | - |
? | |
GMP + sulfite + cytochrome c | - |
Thiobacillus thioparus | guanylyl sulfate + ? | - |
? | |
GMP + sulfite + ferricyanide | - |
Thiobacillus denitrificans | guanylyl sulfate + ferrocyanide | - |
? | |
UMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | uridylyl sulfate + ferrocyanide | - |
? | |
UMP + sulfite + ferricyanide | no activity with UMP | Thiobacillus denitrificans | uridylyl sulfate + ferrocyanide | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thiobacillus denitrificans |
60 | - |
6 min, stable | Thiobacillus thioparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ferricyanide assay, phosphate buffer | Thiobacillus thioparus |
7.2 | - |
- |
Thiobacillus denitrificans |
7.4 | - |
ferricyanide assay, Tris-HCl buffer | Thiobacillus thioparus |
9.5 | - |
reaction with cytochrome c as electron acceptor, Tris-HCl buffer | Thiobacillus thioparus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Thiobacillus thioparus | |
FAD | noncovalently bound | Thiobacillus denitrificans |