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Literature summary for 1.8.98.2 extracted from

  • Iglesias-Baena, I.; Barranco-Medina, S.; Lazaro-Payo, A.; Lopez-Jaramillo, F.J.; Sevilla, F.; Lazaro, J.J.
    Characterization of plant sulfiredoxin and role of sulphinic form of 2-Cys peroxiredoxin (2010), J. Exp. Bot., 61, 1509-1521.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.98.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C72S site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
E76A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Arabidopsis thaliana
K40Q site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
additional information construction of a Srx knockout mutant, phenotype, overview Arabidopsis thaliana
R28Q site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
R28Q/E76A site-directed mutagenesis, inactive mutant Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis, overview Arabidopsis thaliana
0.029
-
 peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate Arabidopsis thaliana
0.079
-
 peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast Srx possesses a chloroplast transit peptide in the N-terminus Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11546
-
 x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana
13914
-
 x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Columbia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type Srx and mutants to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information assay conditions optimization, overview Arabidopsis thaliana ?
-
 ?
additional information assay conditions optimization, overview Arabidopsis thaliana Columbia ?
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
 Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
 Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
 Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
 Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
 Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
 ?
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
 Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
 ?

Subunits

Subunits Comment Organism
? x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Srx
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00042
-
 peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate Arabidopsis thaliana
0.011
-
 peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana mature enzyme, sequence calculation
-
 9.86

General Information

General Information Comment Organism
physiological function the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfinic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx and the conserved Srx is capable of regenerating the functionality of both pea and Arabidopsis Prx-SO2H Arabidopsis thaliana