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Literature summary for 1.8.98.1 extracted from
- Purification of a two-subunit cytochrome-b-containing heterodisulfide reductase from methanol-grown Methanosarcina barkeri (1994), Eur. J. Biochem., 221, 855-861.
General Stability
| General Stability | Organism |
|---|---|
| more stable in presence of CHAPS than in the presence of dodecyl beta-D-maltoside | Methanosarcina barkeri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
coenzyme B | pH 7.0, 37°C | Methanosarcina barkeri |  |
| 0.4 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine | pH 7.0, 37°C, reaction with reduced benzyl viologen | Methanosarcina barkeri | |
| 0.8 | - |
coenzyme M | pH 7.0, 37°C | Methanosarcina barkeri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methanosarcina barkeri | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains 280 nmol of non-heme iron per mg of protein | Methanosarcina barkeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
| 46000 | - |
x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | Methanosarcina barkeri | - |
coenzyme B + coenzyme M | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:methanol-grown cell | - |
Methanosarcina barkeri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coenzyme B + coenzyme M + methylene blue | - |
Methanosarcina barkeri | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanosarcina barkeri | coenzyme B + coenzyme M | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen | - |
Methanosarcina barkeri | coenzyme B + coenzyme M + methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Methanosarcina barkeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | activity with reduced benzyl viologen and N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine increases continously with decreasing pH in the range between pH 7.5 and pH 5.0 | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b | the 23000 Da subunit is the cytochrome b, cytochrome b serves as electron donor for the heterodisulfide reduction | Methanosarcina barkeri | |
| FAD | the enzyme contains 3 mol of FAD per mg of protein | Methanosarcina barkeri | |
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