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Literature summary for 1.8.5.4 extracted from
- Cyanobacterial sulfide-quinone reductase: cloning and heterologous expression (2000), J. Bacteriol., 182, 3336-3344.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Aphanothece halophytica |
| expressed in Escherichia coli BL21(DE3) cells | Pseudanabaena limnetica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2n-nonyl-4-hydroxyquinoline-N-oxide | complete inhibition at 0.001 mM | Aphanothece halophytica |  |
| 2n-nonyl-4-hydroxyquinoline-N-oxide | complete inhibition at 0.001 mM | Pseudanabaena limnetica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
Sulfide | in 10 mM potassium HEPES (pH 7.4), 10 mM MgCl2, 10 mM KCl, temperature not specified in the publication | Aphanothece halophytica | |
| 0.02 | - |
Sulfide | in 10 mM potassium HEPES (pH 7.4), 10 mM MgCl2, 10 mM KCl, temperature not specified in the publication | Pseudanabaena limnetica | |
| 0.04 | - |
plastoquinone-1 | in 10 mM potassium HEPES (pH 7.4), 10 mM MgCl2, 10 mM KCl, temperature not specified in the publication | Aphanothece halophytica | |
| 0.04 | - |
plastoquinone-1 | in 10 mM potassium HEPES (pH 7.4), 10 mM MgCl2, 10 mM KCl, temperature not specified in the publication | Pseudanabaena limnetica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Aphanothece halophytica | 16020 | - |
| membrane | - |
Pseudanabaena limnetica | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
estimated from amino acid sequence | Aphanothece halophytica |
| 48000 | - |
estimated from amino acid sequence | Pseudanabaena limnetica |
| 52000 | - |
SDS-PAGE | Pseudanabaena limnetica |
| 57000 | - |
SDS-PAGE | Aphanothece halophytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aphanothece halophytica | Q9KI50 | - |
- |
| Pseudanabaena limnetica | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfide + plastoquinone-1 | - |
Aphanothece halophytica | sulfur + plastoquinol-1 | - |
? | |
| sulfide + plastoquinone-1 | - |
Pseudanabaena limnetica | sulfur + plastoquinol-1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQR | - |
Aphanothece halophytica |
| SQR | - |
Pseudanabaena limnetica |
| sulfide-quinone reductase | - |
Aphanothece halophytica |
| sulfide-quinone reductase | - |
Pseudanabaena limnetica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aphanothece halophytica | |
| FAD | - |
Pseudanabaena limnetica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aphanothece halophytica | estimated from amino acid sequence | - |
5.6 |
| Pseudanabaena limnetica | estimated from amino acid sequence | - |
7.7 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Aphanothece halophytica | SQR activity is induced for 2 h in the presence of 1.25 mM sulfide and light | up |
| Pseudanabaena limnetica | SQR activity is induced for 2 h in the presence of 1.25 mM sulfide and light | up |
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