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Literature summary for 1.8.5.4 extracted from

  • Zhang, Y.; Cherney, M.M.; Solomonson, M.; Liu, J.; James, M.N.; Weiner, J.H.
    Preliminary X-ray crystallographic analysis of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans (2009), Acta Crystallogr. Sect. F, F65, 839-842.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG 600, 0.1 M bis-Tris pH 5.5, 0.1 M ammonium sulfate Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfide + quinone Acidithiobacillus ferrooxidans
-
 elemental sulfur + quinol
-
 ?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Hi-Trap column chromatography, and Superdex 200 gel filtration Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfide + quinone
-
 Acidithiobacillus ferrooxidans elemental sulfur + quinol
-
 ?

Synonyms

Synonyms Comment Organism
SQR
-
 Acidithiobacillus ferrooxidans
sulfide:quinone oxidoreductase
-
 Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
FAD nonconvalently bound Acidithiobacillus ferrooxidans