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Literature summary for 1.8.5.1 extracted from

  • Zhou, H.; Brock, J.; Liu, D.; Board, P.G.; Oakley, A.J.
    Structural insights into the dehydroascorbate reductase activity of human omega-class glutathione transferases (2012), J. Mol. Biol., 420, 190-203.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of isoform GSTO1 in complex with ascoric acid, to 1.7 A resolution. Ascorbic acid binds in the glutathione site, where the glutamyl moiety of GSH binds and stacks against a conserved aromatic residue, F34 Homo sapiens
crystal structures of isoform GSTO2-2, stabilized through site-directed mutagenesis of cysteine residues to serines and determined at 1.9 A resolution in the presence and absence of glutathione Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construcution of different mutants with containing cysteine-to-serine mutations and/or C-terminal residues deleted, kinetic analysis Homo sapiens
Y34A complete loss of activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
dehydroascorbate mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
0.51
-
dehydroascorbate wild-type, pH 6.9, 30°C Homo sapiens
0.52
-
dehydroascorbate mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C Homo sapiens
0.53
-
dehydroascorbate mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
4.18
-
glutathione mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
4.82
-
glutathione wild-type, pH 6.9, 30°C Homo sapiens
5.97
-
glutathione mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
7.8
-
glutathione mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P78417 isoform GSTO-1
-
Homo sapiens Q9H4Y5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + dehydroascorbate
-
Homo sapiens glutathione disulfide + ascorbate
-
?

Synonyms

Synonyms Comment Organism
GSTO1
-
Homo sapiens
GSTO2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0047
-
dehydroascorbate mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
0.0058
-
glutathione mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
0.008
-
dehydroascorbate mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
0.011
-
dehydroascorbate wild-type, pH 6.9, 30°C Homo sapiens
0.0135
-
dehydroascorbate mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C Homo sapiens
0.0138
-
glutathione mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C Homo sapiens
0.0175
-
glutathione wild-type, pH 6.9, 30°C Homo sapiens
0.0293
-
glutathione mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C Homo sapiens