Crystallization (Comment) | Organism |
---|---|
crystal structures of isoform GSTO1 in complex with ascoric acid, to 1.7 A resolution. Ascorbic acid binds in the glutathione site, where the glutamyl moiety of GSH binds and stacks against a conserved aromatic residue, F34 | Homo sapiens |
crystal structures of isoform GSTO2-2, stabilized through site-directed mutagenesis of cysteine residues to serines and determined at 1.9 A resolution in the presence and absence of glutathione | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construcution of different mutants with containing cysteine-to-serine mutations and/or C-terminal residues deleted, kinetic analysis | Homo sapiens |
Y34A | complete loss of activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
dehydroascorbate | mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
0.51 | - |
dehydroascorbate | wild-type, pH 6.9, 30°C | Homo sapiens | |
0.52 | - |
dehydroascorbate | mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C | Homo sapiens | |
0.53 | - |
dehydroascorbate | mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
4.18 | - |
glutathione | mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
4.82 | - |
glutathione | wild-type, pH 6.9, 30°C | Homo sapiens | |
5.97 | - |
glutathione | mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
7.8 | - |
glutathione | mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P78417 | isoform GSTO-1 | - |
Homo sapiens | Q9H4Y5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + dehydroascorbate | - |
Homo sapiens | glutathione disulfide + ascorbate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GSTO1 | - |
Homo sapiens |
GSTO2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
dehydroascorbate | mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
0.0058 | - |
glutathione | mutant with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
0.008 | - |
dehydroascorbate | mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
0.011 | - |
dehydroascorbate | wild-type, pH 6.9, 30°C | Homo sapiens | |
0.0135 | - |
dehydroascorbate | mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C | Homo sapiens | |
0.0138 | - |
glutathione | mutant containing six cysteine-to-serine mutations, with C-terminal residues FGLC deleted, pH 6.9, 30°C | Homo sapiens | |
0.0175 | - |
glutathione | wild-type, pH 6.9, 30°C | Homo sapiens | |
0.0293 | - |
glutathione | mutant containing six cysteine-to-serine mutations with the C-terminal cysteine residue deleted, pH 6.9, 30°C | Homo sapiens |