Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21 (DE3) strain using the pET-28a(+) expression vector | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Oryza sativa | glutathione disulfide + ascorbate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OsDHAR | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay performed at room temperature | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
physiological function | OsDHAR transformed Escherichia coli BL21 cells show significantly higher DHAR activity and a lower level of reactive oxygen species than the Escherichia coli cells transformed by an empty vector. The DHAR-overexpressing Escherichia coli strain is more tolerant to oxidant- and heavy metalmediated stress conditions than the control Escherichia coli strain, suggesting that the overexpressed rice DHAR gene effectively functions in a prokaryotic system and provides protection to various oxidative stresses | Oryza sativa |