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Literature summary for 1.8.5.1 extracted from

  • Ken, C.F.; Lin, C.Y.; Jiang, Y.C.; Wen, L.; Lin, C.T.
    Cloning, expression, and characterization of an enzyme possessing both glutaredoxin and dehydroascorbate reductase activity from Taiwanofungus camphorata (2009), J. Agric. Food Chem., 57, 10357-10362.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the coding region is expressed as a His-tagged fusion protein in Escherichia coli Taiwanofungus camphoratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.85
-
dehydroascorbate
-
Taiwanofungus camphoratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
calculated from cDNA Taiwanofungus camphoratus
15000
-
SDS-PAGE and electrospray ionization quadrupole-time-of-flight (ESI Q-TOF), His-tagged fusion protein Taiwanofungus camphoratus

Organism

Organism UniProt Comment Textmining
Taiwanofungus camphoratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
functional TcGrx is purified by Ni2+-nitrilotriacetic acid sepharose Taiwanofungus camphoratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
Taiwanofungus camphoratus glutathione disulfide + ascorbate
-
?

Subunits

Subunits Comment Organism
monomer electrospray ionization quadrupole-time-of-flight (ESI Q-TOF), 1 * 15000 Da (His-tagged fusion protein) Taiwanofungus camphoratus

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
Taiwanofungus camphoratus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Taiwanofungus camphoratus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the half-life of deactivation of the enzyme at 100°C is 8.5 min, and its thermal inactivation rate constant Kd is 0.0652/min Taiwanofungus camphoratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.51
-
dehydroascorbate
-
Taiwanofungus camphoratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Taiwanofungus camphoratus

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Taiwanofungus camphoratus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.62
-
dehydroascorbate
-
Taiwanofungus camphoratus