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Literature summary for 1.8.5.1 extracted from

  • Schmuck, E.M.; Board, P.G.; Whitbread, A.K.; Tetlow, N.; Cavanaugh, J.A.; Blackburn, A.C.; Masoumi, A.
    Characterization of the monomethylarsonate reductase and dehydroascorbate reductase activities of Omega class glutathione transferase variants: implications for arsenic metabolism and the age-at-onset of Alzheimers and Parkinsons diseases (2005), Pharmacogenet. Genomics, 15, 493-501.
    View publication on PubMed

Application

Application Comment Organism
medicine significant association with the age-at-onset of Alzheimer's disease and Parkinson's disease Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
A140D no alteration in specific activity compared to the wild type enzyme Homo sapiens
E155 the deletion causes a 2-3fold increase in the specific activity with each substrate and a significant decrease in the enzyme's heat stability, it is also linked to abnormal arsenic excretion patterns Homo sapiens
E208K no alteration in specific activity compared to the wild type enzyme Homo sapiens
N142D no effect on the specific activity of the enzyme with any substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
dehydroascorbate allelic variant D142 of GSTO2-2 Homo sapiens
0.5
-
dehydroascorbate allelic variant N142 of GSTO2-2 Homo sapiens
11.4
-
glutathione allelic variant D142 of GSTO2-2 Homo sapiens
11.8
-
glutathione allelic variant N142 of GSTO2-2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P78417
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-agarose affinity chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
GSTO2-2, using 8 mM urea Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
allelic variant A140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
0.14
-
allelic variant A140/E155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
0.14
-
allelic variant D140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
0.21
-
allelic variant D140/DELTAE155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
0.25
-
allelic variant A140/DELTAE155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
11.6
-
allelic variant D142 of GSTO2-2, dehydroascorbate as substrate, at 30°C Homo sapiens
13.8
-
allelic variant N142 of GSTO2-2, dehydroascorbate as substrate, at 30°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
Homo sapiens GSSG + ascorbate
-
?
glutathione + dehydroascorbate
-
Homo sapiens glutathione disulfide + ascorbate
-
r

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
Homo sapiens
DHA reductase
-
Homo sapiens
DHAR
-
Homo sapiens
GSTO1-1
-
Homo sapiens
GSTO2-2
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
at 53°C, both GSTO2-2 isoforms show a similar loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
Homo sapiens