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Literature summary for 1.8.5.1 extracted from

  • Amako, K.; Ushimaru, T.; Ishikawa, A.; Ogishi, Y.; Kishimoto, R.; Goda, K.
    Heterologous expression of dehydroascorbate reductase from rice and its application to determination of dehydroascorbate concentrations (2006), J. Nutr. Sci. Vitaminol., 52, 89-95.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Cyanate 500 mM, 15% inhibition Oryza sativa
iodoacetic acid 41% and 1% residual activity after treatment with 0.1 mM and 1 mM, respectively Oryza sativa
additional information heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
dehydroascorbate rice recombinant DHAR Oryza sativa
1
-
glutathione rice recombinant DHAR Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Oryza sativa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
theoretical molecular weight calculated from sequence of cDNA Oryza sativa
27000
-
Ni2+-chelating NTA affinity chromatography, doublet polypeptide Oryza sativa
27000
-
SDS-PAGE, doublet polypeptide Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chealting NiNTA Superflow column chromatography Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.1
-
rice cytosolic DHAR Oryza sativa
350
-
rice recombinant DHAR Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
Oryza sativa GSSG + ascorbate
-
?

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
Oryza sativa
DHAR
-
Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the activity of the enzyme at pH 6 was only 20% of that at pH 8.0 Oryza sativa