Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cyanate | 500 mM, 15% inhibition | Oryza sativa | |
iodoacetic acid | 41% and 1% residual activity after treatment with 0.1 mM and 1 mM, respectively | Oryza sativa | |
additional information | heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
dehydroascorbate | rice recombinant DHAR | Oryza sativa | |
1 | - |
glutathione | rice recombinant DHAR | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryza sativa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
theoretical molecular weight calculated from sequence of cDNA | Oryza sativa |
27000 | - |
Ni2+-chelating NTA affinity chromatography, doublet polypeptide | Oryza sativa |
27000 | - |
SDS-PAGE, doublet polypeptide | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chealting NiNTA Superflow column chromatography | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49.1 | - |
rice cytosolic DHAR | Oryza sativa |
350 | - |
rice recombinant DHAR | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Oryza sativa | GSSG + ascorbate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Oryza sativa |
DHAR | - |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the activity of the enzyme at pH 6 was only 20% of that at pH 8.0 | Oryza sativa |