Protein Variants | Comment | Organism |
---|---|---|
C23S | mutant enzyme has almost no activity | Spinacia oleracea |
C26S | turnover number is 57% of the wild-type value, KM-value for dehydroascorbate is 2fold lower than the wild-type value, KM-value for GSH is 1.6old lower than the wild-type value | Spinacia oleracea |
C9S | turnover number is 86% of the wild-type value, KM-value for dehydroascorbate is 2.8fold lower than the wild-type value, KM-value for GSH is 2fold lower than the wild-type value | Spinacia oleracea |
C9S/C26S | turnover number is 43% of the wild-type value, KM-value for dehydroascorbate is 1.1fold higher than the wild-type value, KM-value for GSH is identical to the wild-type value | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
dehydroascorbate | mutant enzyme C9S | Spinacia oleracea | |
0.026 | - |
dehydroascorbate | mutant enzyme C23S | Spinacia oleracea | |
0.053 | - |
dehydroascorbate | wild-type enzyme | Spinacia oleracea | |
0.058 | - |
dehydroascorbate | - |
Spinacia oleracea | |
0.69 | - |
GSH | mutant enzyme C23S | Spinacia oleracea | |
0.95 | - |
GSH | mutant enzyme C9S | Spinacia oleracea | |
1.1 | - |
GSH | wild-type enzyme | Spinacia oleracea | |
1.1 | - |
GSH | mutant enzyme C9S/C26S | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + dehydroascorbate | - |
Spinacia oleracea | GSSG + ascorbate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHAR | - |
Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
dehydroascorbate | - |
Spinacia oleracea | |
210 | - |
GSH | mutant enzyme C9S/C26S | Spinacia oleracea | |
280 | - |
GSH | mutant enzyme C23S | Spinacia oleracea | |
280 | - |
dehydroascorbate | mutant enzyme C23S | Spinacia oleracea | |
420 | - |
GSH | mutant enzyme C9S | Spinacia oleracea | |
420 | - |
dehydroascorbate | mutant enzyme C9S | Spinacia oleracea | |
490 | - |
GSH | wild-type enzyme | Spinacia oleracea | |
490 | - |
dehydroascorbate | wild-type enzyme | Spinacia oleracea |