Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, enhances the activity by 13% | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | 0.1 mM, 48% loss of activity. 1.0 mM, 91% loss of activity | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
dehydroascorbate | - |
Oryza sativa | |
0.84 | - |
GSH | - |
Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 26000, SDS-PAGE | Oryza sativa |
27000 | - |
gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | Oryza sativa | the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells | GSSG + ascorbate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49.1 | - |
- |
Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells | Oryza sativa | GSSG + ascorbate | - |
? | |
GSH + dehydroascorbate | - |
Oryza sativa | GSSG + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, SDS-PAGE | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 12% loss of activity | Oryza sativa |
80 | - |
10 min, 10% loss of activity | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Oryza sativa |