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Literature summary for 1.8.5.1 extracted from

  • Shimaoka, T.; Yokota, A.; Miyake, C.
    Purification and characterization of chloroplast dehydroascorbate reductase from spinach leaves (2000), Plant Cell Physiol., 41, 1110-1118.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
enzyme form DHAR-a, expression in Escherichia coli Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
dehydroascorbate recombinant enzyme form DHAR-1 Spinacia oleracea
0.07
-
dehydroascorbate enzyme form DHAR-a Spinacia oleracea
0.08
-
dehydroascorbate enzyme form DHAR-b Spinacia oleracea
1.1
-
GSH enzyme form DHAR-a and recombinant enzyme form DHAR-a Spinacia oleracea
2.5
-
GSH enzyme form DHAR-b Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma Spinacia oleracea 9507
-
additional information two major dehydroascorbate reductases exist in spinach leaves. One form, DHAR-1, originates in chloroplast, the other, DHAR-b, occurs in a subcellular compartment other than chloroplast Spinacia oleracea
-
-
plastid
-
Spinacia oleracea 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 25000, enzyme form DHAR-b, SDS-PAGE Spinacia oleracea
26000
-
1 * 26000, enzyme form DHAR-a, SDS-PAGE Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
360
-
mitochondrial enzyme Spinacia oleracea
400
-
plastid enzyme Spinacia oleracea

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 week Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSH + dehydroascorbate
-
Spinacia oleracea GSSG + ascorbate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 25000, enzyme form DHAR-b, SDS-PAGE Spinacia oleracea
monomer 1 * 26000, enzyme form DHAR-a, SDS-PAGE Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
10 min, 81% loss of activity of enzyme form DHAR-a, 97% loss of activity of enzyme form DHAR-b Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
enzyme form DHAR-b Spinacia oleracea
7.9
-
enzyme form DHAR-a Spinacia oleracea