Cloned (Comment) | Organism |
---|---|
enzyme form DHAR-a, expression in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
dehydroascorbate | recombinant enzyme form DHAR-1 | Spinacia oleracea | |
0.07 | - |
dehydroascorbate | enzyme form DHAR-a | Spinacia oleracea | |
0.08 | - |
dehydroascorbate | enzyme form DHAR-b | Spinacia oleracea | |
1.1 | - |
GSH | enzyme form DHAR-a and recombinant enzyme form DHAR-a | Spinacia oleracea | |
2.5 | - |
GSH | enzyme form DHAR-b | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma | Spinacia oleracea | 9507 | - |
additional information | two major dehydroascorbate reductases exist in spinach leaves. One form, DHAR-1, originates in chloroplast, the other, DHAR-b, occurs in a subcellular compartment other than chloroplast | Spinacia oleracea | - |
- |
plastid | - |
Spinacia oleracea | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 25000, enzyme form DHAR-b, SDS-PAGE | Spinacia oleracea |
26000 | - |
1 * 26000, enzyme form DHAR-a, SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
mitochondrial enzyme | Spinacia oleracea |
400 | - |
plastid enzyme | Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, stable for at least 1 week | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + dehydroascorbate | - |
Spinacia oleracea | GSSG + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, enzyme form DHAR-b, SDS-PAGE | Spinacia oleracea |
monomer | 1 * 26000, enzyme form DHAR-a, SDS-PAGE | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, 81% loss of activity of enzyme form DHAR-a, 97% loss of activity of enzyme form DHAR-b | Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
enzyme form DHAR-b | Spinacia oleracea |
7.9 | - |
enzyme form DHAR-a | Spinacia oleracea |