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Literature summary for 1.8.4.8 extracted from

  • Bhave, D.P.; Hong, J.A.; Lee, M.; Jang, W.; Krebs, C.; Carroll, K.S.
    Spectroscopic studies on the [4Fe-4S] cluster in adenosine 5-phosphosulfate reductase from Mycobacterium tuberculosis (2011), J. Biol. Chem., 286, 1216-1226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.8

Application

Application Comment Organism
medicine in Mycobacterium tuberculosis, APR is a validated target against the latent phase of infection Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
K144A | the mutation causes a 63000fold increase of kcat/KM and decreases the value of kmax by 270fold. Relative to the wild type enzyme, the affinity of adenosine 5’-phosphosulfate for K144A is decreased by 400fold Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron-sulfur protein, each mole of protein contains 3.3 mol of Fe Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-phosphosulfate + thioredoxin
-
 Mycobacterium tuberculosis AMP + sulfite + oxidized thioredoxin
-
 ?

Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulfate reductase
-
 Mycobacterium tuberculosis
APR
-
 Mycobacterium tuberculosis
APS reductase
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center the enzyme harbors a [4Fe-4S]2+ cluster Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 adenosine 5'-phosphosulfate mutant enzyme K144A, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5 Mycobacterium tuberculosis
2500
-
 adenosine 5'-phosphosulfate wild type enzyme, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5 Mycobacterium tuberculosis