Cloned (Comment) | Organism |
---|---|
expression in strain BL21(DE3) as His10-tagged protein | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
0.0072 | - |
glutaredoxin mutant 1C14S | pH 8.0 | Escherichia coli | |
0.0137 | - |
thioredoxin 1 | pH 8.0 | Escherichia coli | |
0.0149 | - |
glutaredoxin 1 | pH 8.0 | Escherichia coli | |
0.0225 | - |
3'-phosphoadenylyl-sulfate | with cofactors Trx1, Trx2, or Grx1, pH 8.0 | Escherichia coli | |
0.0342 | - |
thioredoxin 2 | pH 8.0 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl-sulfate + thioredoxin | Escherichia coli | enzyme catalyzes the first reductive step in sulfate assimilation | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | Escherichia coli | thioredoxin1 and glutaredoxin 1 are essential for sulfate reduction but not for ribonucleotide reduction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged enzyme from strain BL21(DE3) by chelate affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin | ping pong type reaction mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cofactor specificity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + glutaredoxin 1 | - |
Escherichia coli | adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide | - |
r | |
3'-phosphoadenylyl sulfate + glutaredoxin mutant 1C14S | - |
Escherichia coli | adenosine 3',5'-bisphosphate + sulfite + glutaredoxin mutant 1C14S disulfide | - |
r | |
3'-phosphoadenylyl sulfate + thioredoxin 1 | - |
Escherichia coli | adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide | - |
r | |
3'-phosphoadenylyl sulfate + thioredoxin 2 | - |
Escherichia coli | adenosine 3',5'-bisphosphate + sulfite + thioredoxin 2 disulfide | - |
r | |
3'-phosphoadenylyl-sulfate + glutaredoxin | - |
Escherichia coli | adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | - |
Escherichia coli | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | enzyme catalyzes the first reductive step in sulfate assimilation | Escherichia coli | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | thioredoxin1 and glutaredoxin 1 are essential for sulfate reduction but not for ribonucleotide reduction | Escherichia coli | ? | - |
? | |
additional information | cofactor specificity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30634.4, recombinant enzyme, mass spectrometry | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
3'-Phosphoadenylylsulfate reductase | - |
Escherichia coli |
PAPS reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
3'-phosphoadenylyl-sulfate | pH 9.2-9.3, 35°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | 9.3 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutaredoxin | in vitro, variant Grx1 is active similar to the thioredoxins, while Grx2 and Grx3 are not active, and a mutant form Grx1C14S is a poor cofactor | Escherichia coli | |
thioredoxin | 2 variants Trx1 and Trx2, equally active | Escherichia coli |