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Literature summary for 1.8.4.8 extracted from

  • Lillig, C.H.; Prior, A.; Schwenn, J.D.; Aslund, F.; Ritz, D.; Vlamis-Gardikas, A.; Holmgren, A.
    New thioredoxins and glutaredoxins as electron donors of 3'-phosphoadenylylsulfate reductase (1999), J. Biol. Chem., 274, 7695-7698.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in strain BL21(DE3) as His10-tagged protein Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Escherichia coli
0.0072
-
glutaredoxin mutant 1C14S pH 8.0 Escherichia coli
0.0137
-
thioredoxin 1 pH 8.0 Escherichia coli
0.0149
-
glutaredoxin 1 pH 8.0 Escherichia coli
0.0225
-
3'-phosphoadenylyl-sulfate with cofactors Trx1, Trx2, or Grx1, pH 8.0 Escherichia coli
0.0342
-
thioredoxin 2 pH 8.0 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl-sulfate + thioredoxin Escherichia coli enzyme catalyzes the first reductive step in sulfate assimilation adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
additional information Escherichia coli thioredoxin1 and glutaredoxin 1 are essential for sulfate reduction but not for ribonucleotide reduction ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His10-tagged enzyme from strain BL21(DE3) by chelate affinity chromatography Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin ping pong type reaction mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
cofactor specificity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + glutaredoxin 1
-
Escherichia coli adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide
-
r
3'-phosphoadenylyl sulfate + glutaredoxin mutant 1C14S
-
Escherichia coli adenosine 3',5'-bisphosphate + sulfite + glutaredoxin mutant 1C14S disulfide
-
r
3'-phosphoadenylyl sulfate + thioredoxin 1
-
Escherichia coli adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide
-
r
3'-phosphoadenylyl sulfate + thioredoxin 2
-
Escherichia coli adenosine 3',5'-bisphosphate + sulfite + thioredoxin 2 disulfide
-
r
3'-phosphoadenylyl-sulfate + glutaredoxin
-
Escherichia coli adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
?
3'-phosphoadenylyl-sulfate + thioredoxin
-
Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
3'-phosphoadenylyl-sulfate + thioredoxin enzyme catalyzes the first reductive step in sulfate assimilation Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
additional information thioredoxin1 and glutaredoxin 1 are essential for sulfate reduction but not for ribonucleotide reduction Escherichia coli ?
-
?
additional information cofactor specificity Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 30634.4, recombinant enzyme, mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
3'-Phosphoadenylylsulfate reductase
-
Escherichia coli
PAPS reductase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
3'-phosphoadenylyl-sulfate pH 9.2-9.3, 35°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2 9.3
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
glutaredoxin in vitro, variant Grx1 is active similar to the thioredoxins, while Grx2 and Grx3 are not active, and a mutant form Grx1C14S is a poor cofactor Escherichia coli
thioredoxin 2 variants Trx1 and Trx2, equally active Escherichia coli