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Literature summary for 1.8.4.14 extracted from

  • Kim, H.S.; Kwak, G.H.; Lee, K.; Jo, C.H.; Hwang, K.Y.; Kim, H.Y.
    Structural and biochemical analysis of a type II free methionine-R-sulfoxide reductase from Thermoplasma acidophilum (2014), Arch. Biochem. Biophys., 560, 10-19.
    View publication on PubMed
Search for more literature for 1.8.4.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Crystals from native and mutant enzyme grew at 20°C within 2 days. To obtain a substrate-bound form, the crystals of the C84S mutant enzyme are soaked with the mother liquid containing 10 mM Met-R-O at 22°C for 1 h Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
C15S kcat and Km values of C15S are similar to those of wild type. Cells expressing the C15S mutant enzyme show similar growth to wild type-expressing cells Thermoplasma acidophilum
C60S the kcat value is reduced in the C60S mutant (80fold lower), while the Km value does not significantly change compared to wild type. In cells expressing C60S, growth is not observed during a 3-day incubation but observed at a 7-day incubation Thermoplasma acidophilum
C84S activity is not detectable. Cells expressing C84S do not grow in the methionione sulfoxide medium at all Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.4
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum
0.47
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15924
-
 2 * 15924, calculated from sequence Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HJW5
-
-

Oxidation Stability

Oxidation Stability Organism
cells expressing the wild-type enzyme and C15S mutant enzyme exhibit increased resistance to H2O2-mediated oxidative stress. The resistance is similar to that of the wild type-expressing cells. In contrast, cells expressing both the C84S and C60S mutants show no resistance to the peroxide treatment Thermoplasma acidophilum

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-S-oxide + thioredoxin the enzyme exhibits strict substrate specificity toward free L-methionine (R)-S-oxide and is unable to reduce dabsylated (peptidyl) L-methionine (R)-S-oxide, free L-methionine S-oxide, or dimethylsulfoxide Thermoplasma acidophilum L-methionine + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 15924, calculated from sequence Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
fRMsr
-
 Thermoplasma acidophilum
methionine-R-sulfoxide reductase
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.29
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum
2.8
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.62
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum
0.67
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum