Protein Variants | Comment | Organism |
---|---|---|
C98S | the mutant show increased Km values with dithiothreitol (1.7fold) and thioredoxin (6fold) compared to the wild type enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
dithiothreitol | wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C | Mus musculus | |
0.05 | - |
dithiothreitol | mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C | Mus musculus | |
0.66 | - |
thioredoxin | wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C | Mus musculus | |
7 | - |
thioredoxin | mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q78J03 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
in 100 mM sodium phosphate, at 37°C and pH 5.7 | Mus musculus |
0.055 | - |
in 100 mM sodium phosphate, at 37°C and pH 6.5 | Mus musculus |
0.074 | - |
in 50 mM carbonate-bicarbonate, at 37°C and pH 10.0 | Mus musculus |
0.11 | - |
in 100 mM sodium phosphate, at 37°C and pH 7.0 | Mus musculus |
0.119 | - |
in 50 mM Tris-HCl, at 37°C and pH 7.6 | Mus musculus |
0.228 | - |
in 100 mM sodium phosphate, at 37°C and pH 7.5 | Mus musculus |
0.267 | - |
in 50 mM Tris-HCl, at 37°C and pH 8.0 | Mus musculus |
0.272 | - |
in 50 mM carbonate-bicarbonate, at 37°C and pH 9.6 | Mus musculus |
0.446 | - |
in 50 mM Tris-HCl, at 37°C and pH 8.6 | Mus musculus |
0.462 | - |
in 50 mM carbonate-bicarbonate, at 37°C and pH 9.2 | Mus musculus |
0.484 | - |
in 50 mM Tris-HCl, at 37°C and pH 9.0 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol | - |
Mus musculus | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine (R)-sulfoxide + thioredoxin | - |
Mus musculus | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase B2 | - |
Mus musculus |
MsrB2 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mus musculus |