Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of plastidic isozymes MsrB1 and MsrB2, functional expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
L-methionine-(R)-S-oxide | recombinant isozyme MsrB2, with dithioerythritol | Arabidopsis thaliana | |
0.079 | - |
L-methionine-(R)-S-oxide | recombinant isozyme MsrB1, with dithioerythritol | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastidic isozymes MsrB1 and MsrB2, analysis of subcellular localization | Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine-(R)-S-oxide + thioredoxin | Arabidopsis thaliana | absolute stereospecific reduction | L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
plastidic isozymes MsrB1 and MsrB2 | - |
Purification (Comment) | Organism |
---|---|
recombinant plastidic isozymes MsrB1 and MsrB2 from Escherichia coli | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine-(R)-S-oxide + dithioerythritol | absolute stereospecific reduction, MsrB1 and MsrB2 | Arabidopsis thaliana | L-methionine + dithioerythritol disulfide + H2O | - |
? | |
L-methionine-(R)-S-oxide + thioredoxin | absolute stereospecific reduction | Arabidopsis thaliana | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine-(R)-S-oxide + thioredoxin | absolute stereospecific reduction, isozyme MsrB2, no activity with isozyme MsrB1 | Arabidopsis thaliana | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | substrate specificity, overview, isozyme MsrB1 is not able to reduce free L-methionine-(R)-S-oxide or N-acetyl-L-methionine-(R)-S-oxide, while isozyme MsrB2 prefers protein-bound substrates such as N-acetyl-L-methionine (R)-S-oxide, overview | Arabidopsis thaliana | ? | - |
? | |
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin | protein-bound substrate, preferred substrate of isozyme MsrB2 | Arabidopsis thaliana | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase B | - |
Arabidopsis thaliana |
MsrB | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-methionine-(R)-S-oxide | recombinant isozyme MsrB2, with dithioerythritol | Arabidopsis thaliana | |
0.07 | - |
L-methionine-(R)-S-oxide | recombinant isozyme MsrB1, with dithioerythritol | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | DTE | Arabidopsis thaliana | |
thioredoxin | isozyme MsrB2, no activity with isozyme MsrB1 | Arabidopsis thaliana |