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Literature summary for 1.8.4.12 extracted from

  • Jung, S.; Hansel, A.; Kasperczyk, H.; Hoshi, T.; Heinemann, S.H.
    Activity, tissue distribution and site-directed mutagenesis of a human peptide methionine sulfoxide reductase of type B: hCBS1 (2002), FEBS Lett., 527, 91-94.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of truncated wild-type and mutant enzymes as His-tagged proteins in Escherichia coli, functional coexpression of CBS1 in oocytes with Drosophila melanogaster ShC/B potassium channel Homo sapiens

Protein Variants

Protein Variants Comment Organism
C105S site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Homo sapiens
C169S site-directed mutagenesis, active site mutant, completely inactive mutant Homo sapiens
W110A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
selenium selenoprotein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-methionine (R)-sulfoxide + thioredoxin Homo sapiens type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins protein L-methionine + thioredoxin disulfide
-
 ?
protein-L-methionine (R)-sulfoxide + dithiothreitol Homo sapiens type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins protein-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O the active site selenocysteine SeC169 is essential for enzyme activity Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
heart most abundant in ventricles, interventricular septum, and apex Homo sapiens
-
heart ventricle
-
 Homo sapiens
-
interventricular septum
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
additional information tissue distribution, main expression of CBS1 in muscle tissues, overview Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protein L-methionine (R)-sulfoxide + thioredoxin type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins Homo sapiens protein L-methionine + thioredoxin disulfide
-
 ?
protein-L-methionine (R)-sulfoxide + dithiothreitol type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins Homo sapiens protein-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CBS1
-
 Homo sapiens
MsrB
-
 Homo sapiens
peptide methionine sulfoxide reductase type B
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
dithiothreitol
-
 Homo sapiens
thioredoxin
-
 Homo sapiens