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Literature summary for 1.8.4.11 extracted from

  • Dai, C.; Han, W.; Wang, M.H.
    Two highly homologous methionine sulfoxide reductase A from tomato (Solanum lycopersicum), exhibit distinct catalytic properties (2012), Protein J., 31, 285-292.
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
C180S the mutant of isoform E4 shows wild type activity Solanum lycopersicum
C180S the mutant of isoform MSRA2 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
C188S the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
C194S the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
C37S inactive Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00322
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.00343
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.0189
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.0218
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.02265
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.0233
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 x * 22000, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin Solanum lycopersicum
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum G3JX11 cultivar Micro-Tom
-
Solanum lycopersicum P54153 cultivar Micro-Tom
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-
leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin
-
 Solanum lycopersicum L-methionine + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase A
-
 Solanum lycopersicum
MSRA2
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.1
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.11
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.13
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.15
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
0.17
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Solanum lycopersicum

Expression

Organism Comment Expression
Solanum lycopersicum the transcript level of isoform MSRA2 is significantly accumulated in both leaves and green fruits after ethephon treatment up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
4.9
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
5.1
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
6.9
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
46.5
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
49.5
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum