Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli | substrate is oxidized ribosomal L12 protein, stereospecific reduction | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli Z19 | substrate is oxidized ribosomal L12 protein, stereospecific reduction | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli | oxidized Met-enkephalin | Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli Z19 | oxidized Met-enkephalin | Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli Z19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + DTT | protein-bound substrate, stereospecific reduction, substrate is oxidized ribosomal L12 protein | Escherichia coli | peptide-L-methionine + DTT disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + DTT | protein-bound substrate, stereospecific reduction, substrate is oxidized ribosomal L12 protein | Escherichia coli Z19 | peptide-L-methionine + DTT disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | substrate is oxidized ribosomal L12 protein, stereospecific reduction | Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | protein-bound substrate, stereospecific reduction, substrates are oxidized ribosomal L12 protein or oxidized Met-enkephalin | Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | substrate is oxidized ribosomal L12 protein, stereospecific reduction | Escherichia coli Z19 | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | protein-bound substrate, stereospecific reduction, substrates are oxidized ribosomal L12 protein or oxidized Met-enkephalin | Escherichia coli Z19 | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + DTT | oxidized Met-enkephalin | Escherichia coli | Tyr-Gly-Gly-Phe-L-methionine + DTT disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + DTT | oxidized Met-enkephalin | Escherichia coli Z19 | Tyr-Gly-Gly-Phe-L-methionine + DTT disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin | oxidized Met-enkephalin | Escherichia coli | Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin | oxidized Met-enkephalin | Escherichia coli Z19 | Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetSO-L12 reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Escherichia coli | |
thioredoxin | - |
Escherichia coli |