Cloned (Comment) | Organism |
---|---|
epression of His-tagged enzyme in Escherichia coli | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | - |
Sinorhizobium meliloti | |
additional information | not inhibited by 3'-AMP | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | 0.004 | adenosine-5'-phosphosulfate | - |
Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + thioredoxin | Rhizobium sp. | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Agrobacterium tumefaciens | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Sinorhizobium fredii | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Rhizobium leguminosarum | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Sinorhizobium meliloti | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Rhizobium sp. | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Agrobacterium tumefaciens | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Sinorhizobium fredii | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Rhizobium leguminosarum | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Sinorhizobium meliloti | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Rhizobium sp. NGR234 | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Rhizobium sp. NGR234 | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Rhizobium leguminosarum | - |
- |
- |
Rhizobium sp. | - |
- |
- |
Rhizobium sp. NGR234 | - |
- |
- |
Sinorhizobium fredii | - |
also known as Sinorhizobium fredii | - |
Sinorhizobium meliloti | P56891 | also known as Sinorhizobium meliloti | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme, Ni-nitrilotriacetic acid column | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Rhizobium sp. | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Agrobacterium tumefaciens | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Sinorhizobium fredii | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Rhizobium leguminosarum | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Sinorhizobium meliloti | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Rhizobium sp. | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Agrobacterium tumefaciens | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Sinorhizobium fredii | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Rhizobium leguminosarum | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Sinorhizobium meliloti | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Rhizobium sp. NGR234 | AMP + sulfite + thioredoxin disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Rhizobium sp. NGR234 | AMP + sulfite + thioredoxin disulfide | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Rhizobium sp. | |
thioredoxin | - |
Agrobacterium tumefaciens | |
thioredoxin | - |
Sinorhizobium fredii | |
thioredoxin | - |
Rhizobium leguminosarum | |
thioredoxin | activity drops approx. 5fold in the absence of thioredoxin, glutathione can not replace thioredoxin | Sinorhizobium meliloti |