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Literature summary for 1.8.4.10 extracted from

  • Abola, A.P.; Willits, M.G.; Wang, R.C.; Long, S.R.
    Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae (1999), J. Bacteriol., 181, 5280-5287.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
epression of His-tagged enzyme in Escherichia coli Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
5'-AMP
-
Sinorhizobium meliloti
additional information not inhibited by 3'-AMP Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003 0.004 adenosine-5'-phosphosulfate
-
Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-adenylyl sulfate + thioredoxin Rhizobium sp. no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Agrobacterium tumefaciens no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Sinorhizobium fredii no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Rhizobium leguminosarum no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Sinorhizobium meliloti no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Rhizobium sp. involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Agrobacterium tumefaciens involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Sinorhizobium fredii involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Rhizobium leguminosarum involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Sinorhizobium meliloti involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Rhizobium sp. NGR234 no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin Rhizobium sp. NGR234 involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Rhizobium leguminosarum
-
-
-
Rhizobium sp.
-
-
-
Rhizobium sp. NGR234
-
-
-
Sinorhizobium fredii
-
also known as Sinorhizobium fredii
-
Sinorhizobium meliloti P56891 also known as Sinorhizobium meliloti
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme, Ni-nitrilotriacetic acid column Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium sp. AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Agrobacterium tumefaciens AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Sinorhizobium fredii AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium leguminosarum AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Sinorhizobium meliloti AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium sp. AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Agrobacterium tumefaciens AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Sinorhizobium fredii AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium leguminosarum AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Sinorhizobium meliloti AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium sp. NGR234 AMP + sulfite + thioredoxin disulfide
-
?
5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium sp. NGR234 AMP + sulfite + thioredoxin disulfide
-
?

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Rhizobium sp.
thioredoxin
-
Agrobacterium tumefaciens
thioredoxin
-
Sinorhizobium fredii
thioredoxin
-
Rhizobium leguminosarum
thioredoxin activity drops approx. 5fold in the absence of thioredoxin, glutathione can not replace thioredoxin Sinorhizobium meliloti