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Literature summary for 1.8.3.3 extracted from

  • Kusakaba, H.; Kuninaka, A.; Yoshino, H.
    Purification and properties of a new enzyme, glutathione oxidase from Penicillium sp. K-6-5 (1982), Agric. Biol. Chem., 46, 2057-2067.
No PubMed abstract available
Search for more literature for 1.8.3.3

Activating Compound

Activating Compound Comment Organism Structure
EDTA activates slightly Penicillium sp.

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 slight inhibition Penicillium sp.
KCl slight inhibition Penicillium sp.
KI slight inhibition Penicillium sp.
MnSO4 slight inhibition Penicillium sp.
additional information EDTA has no effect on oxidation reaction Penicillium sp.
NiCl2 slight inhibition Penicillium sp.
ZnSO4 complete inhibition at 1 mM Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 glutathione
-
 Penicillium sp.
3.6
-
 L-cysteine
-
 Penicillium sp.
6.7
-
 dithiothreitol
-
 Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium sp.
-
-
intracellular
-
 Penicillium sp. 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
AlCl3 activates slightly Penicillium sp.
CaCl2 activates slightly Penicillium sp.
FeCl2 activates slightly Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-PAGE Penicillium sp.
95000
-
 gel filtration Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + O2 Penicillium sp.
-
 glutathione disulfide + H2O2
-
 ?
glutathione + O2 Penicillium sp. K-6-5
-
 glutathione disulfide + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
 K-6-5
-
Penicillium sp. K-6-5
-
 K-6-5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
272
-
-
 Penicillium sp.

Storage Stability

Storage Stability Organism
-20°C, 0.1 M, potassium phosphate buffer, pH 7.4, 6 months Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol + O2 slowly Penicillium sp. mercaptoethanol disulfide
-
 r
cysteamine + O2 slowly Penicillium sp. ?
-
 ?
D-cysteine + O2 slowly Penicillium sp. D-cystine
-
 ?
dithiothreitol + O2 low activity Penicillium sp. ?
-
 ?
glutathione + O2
-
 Penicillium sp. glutathione disulfide + H2O2
-
 ?
glutathione + O2
-
 Penicillium sp. K-6-5 glutathione disulfide + H2O2
-
 ?
L-cysteine + O2
-
 Penicillium sp. L-cystine
-
 ?
L-cysteine + O2
-
 Penicillium sp. K-6-5 L-cystine
-
 ?
L-cysteine methyl ester + O2 slowly Penicillium sp. L-cystine dimethyl ester
-
 ?
L-cysteine methyl ester + O2 slowly Penicillium sp. K-6-5 L-cystine dimethyl ester
-
 ?
additional information no other amio acids but D- and L-cysteine serve as substrate Penicillium sp. ?
-
 ?
additional information no other amio acids but D- and L-cysteine serve as substrate Penicillium sp. K-6-5 ?
-
 ?
N-acetyl-L-cysteine + O2 slowly Penicillium sp. N,N'-diacetyl-L-cystine
-
 ?
N-acetyl-L-cysteine + O2 slowly Penicillium sp. K-6-5 N,N'-diacetyl-L-cystine
-
 ?
reduced RNase A + O2
-
 Penicillium sp. ?
-
 ?
thiophenol + O2 slowly Penicillium sp. diphenyl sulfide
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, no loss of activity Penicillium sp.
75
-
 30 min, complete loss of activity Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.8
-
 Penicillium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 minimal activity below pH 5.0, active at least up to pH 10.0 Penicillium sp.
6.2 8.8 sharp decline in activity below pH 6.2 and above pH 8.8 Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 8.6 45°C, 30 min Penicillium sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Penicillium sp.
FAD contains 2 mol of FAD per mol of enzyme Penicillium sp.