Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA has no effect on oxidation reaction | Rattus norvegicus | |
thiols | at high concentrations above 20 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
dithiothreitol | - |
Rattus norvegicus | |
4.4 | - |
glutathione | - |
Rattus norvegicus | |
9.1 | - |
L-cysteine | - |
Rattus norvegicus | |
32 | - |
2-mercaptoethanol | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seminal vesicle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-mercaptoethanol + O2 | - |
Rattus norvegicus | mercaptoethanol disulfide | - |
? | |
dithiothreitol + O2 | - |
Rattus norvegicus | ? | - |
? | |
glutathione + O2 | - |
Rattus norvegicus | glutathione disulfide + H2O2 | - |
? | |
L-cysteine + O2 | - |
Rattus norvegicus | L-cystine | - |
? | |
additional information | not: D,L-homocysteine | Rattus norvegicus | ? | - |
? | |
reduced RNase A + O2 | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3.5 min, 50% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate dithioerythritol | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | minimal activity below pH 5.0, active at least up to pH 10.0 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rattus norvegicus | |
FAD | 1 mol FAD per mol of enzyme | Rattus norvegicus |