Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant liver enzymes in Escherichia coli strain BL21(DE3) with a longer or shorter linker His-tag, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C124A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
C15A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Homo sapiens |
C15A/C124A | site-directed mutagenesis, decreased activity compared to the wild-type enzyme | Homo sapiens |
C15A/C74A/C85A/C124A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Homo sapiens |
C74A/C85A | site-directed mutagenesis, decreased activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | redox potential of wild-type and mutant enzymes | Homo sapiens | |
1.3 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A | Homo sapiens | |
1.7 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C74A/C85A/C124A | Homo sapiens | |
1.8 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C124A | Homo sapiens | |
2 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutants C74A/C85A and C15A/C124A | Homo sapiens | |
2.1 | - |
dithiothreitol | pH 7.5, 25°C, recombinant wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme might communicate with the respiratory chain via the mediation of cytochrome c | ? | - |
? | |
R-SH + O2 | Homo sapiens | - |
R-S-S-R + H2O2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant liver enzymes from Escherichia coli strain BL21(DE3) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + O2 | the enzyme forms large amounts of neutral semiquinone, which arises between flavin centers within the dimer, during aerobic turnover with DTT | Homo sapiens | dithiothreitol disulfide + H2O2 | - |
ir | |
dithiothreitol + reduced cytochrome c | cytochrome c is about 100fold more effective than O2 as reducing cosubstrate | Homo sapiens | dithiothreitol disulfide + oxidized cytochrome c | - |
? | |
additional information | enzyme might communicate with the respiratory chain via the mediation of cytochrome c | Homo sapiens | ? | - |
? | |
R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
ir | |
R-SH + O2 | disulfide bridge C15-C124 is not required for activity | Homo sapiens | R-S-S-R + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | monomers are linked via a disulfide bridge C15-C124, which is not critical for dimer formation, structure modeling using the enzymes crystal structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ALR | augmenter of liver regeneration | Homo sapiens |
hepatopoietin | - |
Homo sapiens |
More | enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family | Homo sapiens |
QSOX | - |
Homo sapiens |
quiescin-like flavin-dependent sulfhydryl oxidase | - |
Homo sapiens |
sulfhydryl oxidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C74A/C85A | Homo sapiens | |
0.87 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A | Homo sapiens | |
0.9 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C124A | Homo sapiens | |
0.95 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C124A | Homo sapiens | |
1.1 | - |
dithiothreitol | pH 7.5, 25°C, recombinant wild-type enzyme | Homo sapiens | |
1.25 | - |
dithiothreitol | pH 7.5, 25°C, recombinant mutant C15A/C74A/C85A/C124A | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Homo sapiens |